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The TA is a type of screw drive that uses a triangle-shaped recess in the screw head. This drive can restrict access to the device internals but can readily be driven with hex keys. These screws are often found in children's toys from fast food restaurants, as well as vacuum cleaners, fan heaters, elevators, camping stoves, golf clubs, Breville kettles and Master Locks, among others, Sizes include TA14, TA18, TA20, TA23 and TA27. Note that the sides of the triangle are straight, which differs from Tri-point-3 fasteners.
Polymerizing microtubules exert substantial pushing forces after contact with stiff surfaces44. As microtubules did not grow into condensates of purified chromatin fragments in vitro, we wondered whether the surface tension of liquified endogenous chromatin is strong enough to allow microtubule-based pushing. If this were the case, then droplets of digested chromosomes should be pushed away by polar ejection forces of growing astral microtubules. To test this hypothesis, we injected AluI into mitotic cells treated with nocodazole and then washed out the nocodazole to induce microtubule polymerization, while applying STLC to induce a monopolar astral spindle geometry. Time-lapse imaging showed that, initially, all chromatin resided in a single droplet at the cell centre but, soon after nocodazole washout, the chromatin split into several droplets that moved away from growing microtubule asters (Extended Data Fig. 10a,b). Thus, liquid chromatin condensates can be pushed by polymerizing astral microtubules.
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